NT-PROBNP assay and MINE score in dogs with mitral valve disease
DOI:
https://doi.org/10.13129/1828-6550/%25xAbstract
Myxomatous mitral valve disease (MMVD) is the most common acquired cardiac disease in dogs. This study aimed to evaluate the relationship between the Mitral INsufficiency Echocardiographic (MINE) score, an echocardiographic staging system, and plasma NT-proBNP levels. Twenty-one client-owned dogs with MMVD were prospectively enrolled and underwent clinical, radiographic, electrocardiographic, and echocardiographic examinations. Dogs were classified according to MINE score. Plasma NT-proBNP concentrations were measured using a validated immunoassay. Results showed significant differences in echocardiographic parameters among severity groups and demonstrated a correlation between disease severity and NT-proBNP levels. These findings suggest that combining the MINE score with NT-proBNP measurement may improve staging accuracy, the association with MMVD severity, and clinical management of dogs affected by MMVD
References
1. Haggstrom, J, Kvart, C, Pedersen, HD. (2005). Acquired valvular disease. In: Ettinger SJ, Feldman EC, editors. Textbook of Veterinary Internal Medicine. 6th ed. St. Louis, Elsevier Saunders, 1022–1039.
2. Olsen, LH, Fredholm, M, Pedersen, HD. (1999). Epidemiology and Inheritance of Mitral Valve Prolapse in Dachshunds. J Vet Intern Med, 13(5), 448–56. doi: 10.1111/j.1939-1676.1999.tb01462.x
3. Borgarelli, M, Häggström, J. (2010). Canine degenerative myxomatous mitral valve disease: natural history, clinical presentation and therapy. Vet Clin North Am Small Anim Pract, 2010, 40, 651-663.
4. Aupperle, H, Disatian, S. (2012). Pathology, protein expression and signaling in myxomatous mitral valve degeneration: comparison of dogs and humans. J Vet Cardiol, 14(1), 59‐71.
5. Fox, PR. (2012). Pathology of myxomatous mitral valve disease in the dog. J Vet Cardiol, 14, 103-126.
6. Han, RI, Black, A, Culshaw, GJ, French, AT, Else, RW, Corcoran, BM. (2008). Distribution of myofibroblasts, smooth muscle‐like cells, macrophages, and mast cells in mitral valve leaflets of dogs with myxomatous mitral valve disease. Am J Vet Res, 69, 763‐769.
7. Keene, BW, Atkins, CE, Bonagura, JD, Fox, PR, Häggström, J, Fuentes, VL, Oyama, MA, Rush JE, Stepien, R, Uechi, M. (2019). ACVIM consensus guidelines for the diagnosis and treatment of myxomatous mitral valve disease in dogs. J Vet Intern Med, 33(3), 1127-1140. doi: 10.1111/jvim.15488.
8. Wolf, J, Gerlach, N, Weber, K, Klima, A, Wess, G. (2013). The diagnostic relevance of NT-proBNP and proANP 31-67 measurements in staging of myxomatous mitral valve disease in dogs. Vet Clin Pathol, 42(2), 196-206. doi: 10.1111/vcp.12044.
9. Vezzosi, T, Grosso, G, Tognetti, R, Meucci, V, Patata, V, Marchesotti, F, Domenech, O. (2021). The Mitral INsufficiency Echocardiographic score: A severity classification of myxomatous mitral valve disease in dogs. J Vet Intern Med. 20, 35(3), 1238-1244. doi: 10.1111/jvim.16131.
10. Vezzosi, T, Grosso, G, Vatne, L, Porciello, F, Dall'Aglio, E, Guglielmini, C, Broch, H, Dickson, D, Croce, M, Patata, V, Marchesotti, F, Tognetti, R, Rishniw, M, Domenech O. Risk Stratification Using Mitral INsufficiency Echocardiographic Score 2 in Dogs With Preclinical Mitral Valve Disease. J Vet Intern Med, 39, 5, e70215, https://doi.org/10.1111/jvim.70215.
11. Fox, PR, Oyama, MA, Hezzell, MJ, Rush, JE, Nguyenba, TP, DeFrancesco, TC, Lehmkuhl, LB, Kellihan, HB, Bulmer, B, Gordon, SG, Cunningham, SM, MacGregor, J, Stepien, RL, Lefbom ,B, Adin, D, Lamb, K. (2015). Relationship of plasma N-terminal pro-brain natriuretic peptide concentrations to heart failure classification and cause of respiratory distress in dogs using a 2nd generation ELISA assay. J Vet Intern Med, 29(1), 171-179.
12. de Lima, GV, Ferreira, FDS. (2017) N-terminal-pro brain natriuretic peptides in dogs and cats: A technical and clinical review. Vet World, 10(9), 1072-1082. doi: 10.14202/vetworld.2017.1072-1082.
13. Ogawa, M, Hori, Y, Kanno, N, Iwasa, N, Toyofuku, T, Isayama, N, Yoshikawa, A, Akabane, R, Sakatani, A, Miyakawa, H, Hsu, HH, Miyagawa, Y, Takemura, N. (2021). Comparison of N-terminal pro-atrial natriuretic peptide and three cardiac biomarkers for discriminatory ability of clinical stage in dogs with myxomatous mitral valve disease. J Vet Med Sci, 24, 83(4), 705-715. doi: 10.1292/jvms.20-0629.
14. Oyama, MA, Fox, PR, Rush, JE, Rozanski ,EA, Lesser, M. (2008). Clinical utility of serum N-terminal pro-B-type natriuretic peptide concentration for identifying cardiac disease in dogs and assessing disease severity. J Am Vet Med Assoc, 232, 1496-1503.
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Copyright (c) 2026 Pietro Gambadauro, Giorgio Cavallo, Diego Antonio Sicuso, Vito Biondi, Annamaria Passantino, Michela Pugliese
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