Blood flow cytometry in Mugil cephalus and Carassius auratus: a comparative study

Authors

  • Vincenzo Parrino Università degli Studi di Messina Dipartimento di Scienze Chimica, Biologiche, Farmaceutiche ed Ambientali Viale F. Stagno D’Alcontres 31, 98166 Messina
  • Tiziana Capello Università degli Studi di Messina, Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Viale F. Stagno D’Alcontres 31, 98166 Messina
  • Gregorio Costa Università degli Studi di Messina, Dipartimento di Patologia Umana dell’Adulto e dell’Età Evolutiva, Via Consolare Valeria 2, 98125 Messina
  • Carmela Cannavà Università degli Studi di Messina, Dipartimento di Patologia Umana dell’Adulto e dell’Età Evolutiva, Via Consolare Valeria 2, 98125 Messina
  • Marilena Sanfilippo Università degli Studi di Messina, Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Viale F. Stagno D’Alcontres 31, 98166 Messina
  • Francesco Fazio Università degli Studi di Messina, Dipartimento di Scienze Veterinarie, Polo Universitario dell’Annunziata, 98168 Messina
  • Giuseppe Piccione Università degli Studi di Messina, Dipartimento di Scienze Veterinarie, Polo Universitario dell’Annunziata, 98168 Messina
  • Salvatore Fasulo Università degli Studi di Messina, Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Viale F. Stagno D’Alcontres 31, 98166 Messina

DOI:

https://doi.org/10.1478/AAPP.97S2A15

Abstract

Blood and its parameters can give specific indications on the welfare of fishes.Many endogenous and exogenous factors exert influences on the characteristics of blood. The correct interpretation of fish hematology for a given species depends on the availability of reference values. The purpose of the present study was therefore to build a database with data on haematological profiles of Mugil cephalus (Linnaeus 1758) and goldfish Carassius auratus (Linnaeus 1758), in particular of some blood cells, namely red blood cells (RBC), white blood cells (WBC) and thrombocytes (TC). All the blood parameters studied showed significant differences in the two fish species considered, using flow cytometry and optical microscopy coupled with an automated system. In particular, RBC showed an increase in mullets in respect to goldfish, while WBC and TC decreased. The differences found may be due to the environmental conditions and the different eating habits of the two species. The results of this research will allow to better understand how the different dietary habits and environmental conditions can influence the haematological parameters of fishes. Flow cytometry represents a modern diagnostic technique in human. Moreover, the technique used by combining flow cytometry with automated haematological counting, has proved very effective in the early evaluation of haematological parameters of various fish species.

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Published

2019-12-20

Issue

Vol 97, SUPPL NO 2 (2019): NACS 2017

Section

NACS 2017 (Conference Proceedings)

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